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The customer shall cooperate with VWR in all matters relating to the services, provide all such access and information as is necessary and obtain any licences permissions and consents required before commencement of the services. Tissue-Tek O.C.T. is an embedding compound for cryosectioning, which is soluble in water. It mainly consists of glycols and synthetic resins. Tissue-Tek O.C.T. is used as matrix for cryosectioning of tissues. Using the O.C.T. the tissue samples can be positioned more easily in the microtome and have better qualities during sectioning.
In the rare case that trace amounts of genomic DNA are still detectable in sensitive downstream applications such as e.g.,realtime RT-PCR, an in-solution digest using the RNase-Free DNAase set can be performed. Instructionsare presentedin Appendix C of the RNeasy MinElute Cleanup Handbook. The PureYield™ Plasmid Maxiprep System purifies up to 1mg of transfection-quality plasmid DNA from 250ml bacterial cultures in under an hour. The system is designed for use with a vacuum source and vacuum manifold, greatly reducing the time spent on purification compared to silica resin or other membrane-based methods. Note that the above stepsare suggestions,rather thanofficial protocol recommendations. Please try a "pilot" run on a test sample first. Note that the A260/A280 ratio is influenced considerably by pH. As water is unbuffered, the pH and the resulting 260/280 ratio can vary greatly. For an accurate determination of purity, we recommend measuring the 260/280 absorbance in 10 mM Tris-Cl, pH 7.5. Be sure to calibrate the spectrophotometer with the same solution. Pure RNA has an A260/A280 ratio of 1.9-2.1. However, values up to 2.3 are routinely obtained for pure RNA (in 10 mM Tris, pH 7.5) with some spectrophotometers.
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If the cells in RNAprotect Tissue Reagent cannot be collected by centrifugation, please try one of the following suggestions: On termination of the contract for any reason the customer shall immediately pay to VWR all of its outstanding unpaid invoices and interest. Confidentiality VWR may at any time, without limiting any other rights and remedies that it may have, set off any amount owing to it by the customer under the contract against any amount payable by VWR to the customer (whether under the contract or a separate agreement). Delivery
Customers who exceed their credit limits will be asked to pay in advance for additional products and/or services until the account is settled. The customer is responsible for unloading and transporting large and/or heavy items from delivery vans and for supervising the unloading of all other products delivered. Any liability accepted by VWR under this contract is in lieu of any terms implied by law as to the quality or fitness for any particular purpose of the products and/or the standard of the services and all such implied terms are, to the fullest extent permitted by law, excluded from the contract between VWR and the customer. The customer shall indemnify VWR against any claims made against VWR by the customer’s employees, contractors or agents. Intellectual property rightsFor very low-copy plasmids, expected yields are 20–100 µg for the QIAGEN-tip 100, and 100–500 µg for the QIAGEN-tip 500. An example of the calculations involved in RNA quantification is shown below. Use the buffer in which the RNA is diluted to zero the spectrophotometer: Nothing in this contract shall limit or exclude VWR’s liability for death or personal injury caused by its negligence, fraud, fraudulent misrepresentation, or any other matter in respect of which it would be unlawful for VWR to exclude or restrict liability. Subject to this, in view of the responsibilities of the customer set out in the above paragraphs:
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